Antitumor Effect In Vivo and the Impact of Alkalization Using Sodium Bicarbonate on Viability and Metabolism of CT26 Tumor Ce

In this article, the impact of alkalization using sodium bicarbonate on the survival and growth of tumor cells is examined, 
which is of significant importance for cancer therapy. The studies confirm that the acidic tumor microenvironment is a 
critical factor in cancer progression, facilitating invasion and metastasis while also suppressing immune responses.
In vivo experiments on animals with transplanted colon adenocarcinoma CT26 revealed that parenteral administration 
of a 4% sodium bicarbonate solution leads to systemic alkalization. Although it does not increase the median survival 
compared to the control group, it can results in the inhibition and even regression of tumor growth in some animals. In 
contrast, oral administration of sodium bicarbonate does not lead to systemic alkalization and does not exert such effects.
In vitro studies demonstrated that the application of sodium bicarbonate results in reduced cell proliferation and 
increased cytotoxicity. Alkalization of the medium presumably activates autophagy and significantly decreases the migratory 
potential of tumor cells. These metabolic changes may weaken their adaptation to the acidic environment and enhance 
the effectiveness of standard treatment methods.
The results obtained highlight the potential of alkalizing therapy as an adjunct to traditional cancer treatment methods. 
Further research in this area may lead to a deeper understanding of the molecular and cellular mechanisms associated with 
alkalization and open new avenues for developing effective therapeutic strategies against oncological diseases.